Identification of rosmarinic acid and sulfated flavonoids as inhibitors of microfouling on the surface of eelgrass Zostera marina.

Guan, Chi, Parrot, Delphine, Wiese, Jutta , Sönnichsen, Frank, Saha, Mahasweta , Tasdemir, Deniz and Weinberger, Florian (2017) Identification of rosmarinic acid and sulfated flavonoids as inhibitors of microfouling on the surface of eelgrass Zostera marina. Biofouling, 10 . pp. 867-880. DOI 10.1080/08927014.2017.1383399.

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Abstract

A bioassay-guided approach was used to identify defense compounds that are present on the surface of Zostera marina and which inhibit settlement of microfoulers at natural concentrations. Moderately polar eelgrass surface extracts inhibited the settlement of seven marine bacteria and one yeast that originated from non-living substrata. In contrast, five other bacterial strains that had been directly isolated from eelgrass surfaces were all insensitive, which suggested a selective effect of surface metabolites on the microbial communities present on eelgrass. Bioassay-guided isolation of active compounds from the extracts in combination with UPLC-MS and 1H-NMR spectroscopy resulted in the identification of rosmarinic acid, luteolin-7-sulfate and diosmetin-7-sulfate or its isomer chrysoeriol-7-sulfate. All three compounds are nontoxic repellents, as they did not inhibit bacterial growth, but prevented bacterial settlement in a dose-dependent manner. Between 15.6 and 106.8 μg ml−1 of rosmarinic acid were present on the eelgrass surface, enough for half maximal settlement inhibition of bacteria.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > Applied R&D > GEOMAR-Biotech
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1080/08927014.2017.1383399
ISSN: 0892-7014
Projects: Future Ocean
Date Deposited: 06 Sep 2017 09:50
Last Modified: 01 Feb 2019 15:00
URI: http://oceanrep.geomar.de/id/eprint/39264

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