Chemical analysis of the Alphaproteobacterium strain MOLA1416 associated with the marine lichen Lichina pygmaea.

Parrot, Delphine, Intertaglia, Laurent, Jehan, Philippe, Grube, Martin, Suzuki, Marcelino T. and Tomasi, Sophie (2018) Chemical analysis of the Alphaproteobacterium strain MOLA1416 associated with the marine lichen Lichina pygmaea. Phytochemistry, 145 . pp. 57-67. DOI 10.1016/j.phytochem.2017.10.005.

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Supplementary data:

Abstract

Alphaproteobacterium strain MOLA1416, related to Mycoplana ramosa DSM 7292 and Chelativorans intermedius CC-MHSW-5 (93.6% 16S rRNA sequence identity) was isolated from the marine lichen, Lichina pygmaea and its chemical composition was characterized by a metabolomic network analysis using LC-MS/MS data. Twenty-five putative different compounds were revealed using a dereplication workflow based on MS/MS signatures available through GNPS (https://gnps.ucsd.edu/). In total, ten chemical families were highlighted including isocoumarins, macrolactones, erythrinan alkaloids, prodiginines, isoflavones, cyclohexane-diones, sterols, diketopiperazines, amino-acids and most likely glucocorticoids. Among those compounds, two known metabolites (13 and 26) were isolated and structurally identified and metabolite 26 showed a high cytotoxic activity against B16 melanoma cell lines with an IC50 0.6 ± 0.07 μg/mL.

Document Type: Article
Keywords: Lichen-associated bacteria; Lichina pygmaea; Alphaproteobacteria; LC/MSn; Dereplication workflow; Molecular networking
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.phytochem.2017.10.005
ISSN: 0031-9422
Date Deposited: 22 Nov 2017 10:36
Last Modified: 01 Feb 2019 15:03
URI: http://oceanrep.geomar.de/id/eprint/40207

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