Time Course Effect of R-Alpha-Lipoic Acid on Cellular Metabolomics in Cultured Hepatoma Cells.

Ikuta, Naoko, Chikamoto, Keita, Asano, Yuya, Yasui, Yoshiaki, Yokokawa, Haruka, Terao, Keiji, Rimbach, Gerald and Matsugo, Seiichi (2017) Time Course Effect of R-Alpha-Lipoic Acid on Cellular Metabolomics in Cultured Hepatoma Cells. Journal of Medicinal Food, 20 (3). pp. 211-222. DOI 10.1089/jmf.2016.3837.

Full text not available from this repository.

Supplementary data:

Abstract

Alpha-lipoic acid (LA) is a powerful antioxidant. LA has two enantiomers, R(+)-LA (R-LA) and S(-)-LA (S-LA). Of these, R-LA is naturally occurring and an essential cofactor in energy metabolism. R-LA treatment has been reported to affect glucose metabolism in rat hepatoma cells. This study analyzed the time course of metabolite levels in LA-treated cultured H4IIEC3 rat hepatoma cells, including a specific evaluation of the effect of R-LA and the enantioselectivity of LA. Principal component analysis showed that this experiment was well designed to observe enantioselectivity. R-LA treatment was found to inhibit the glycolysis and Thr-Gly-Ser pathways, as well as lactic acid production, leading to the inhibition of gluconeogenesis in starved H4IIEC3 cells. This study may provide mechanistic insight into how R-LA induces apoptosis in hepatoma cells.

Document Type: Article
Keywords: H4IIEC3; amino acids; enantioselectivity; glyconeogenesis; hepatoma cells; lipoic acid; metabolomics
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1089/jmf.2016.3837
ISSN: 1096-620X
Projects: Future Ocean
Date Deposited: 19 Dec 2017 15:37
Last Modified: 12 Apr 2019 15:24
URI: http://oceanrep.geomar.de/id/eprint/40951

Actions (login required)

View Item View Item