Extraction of dissolved fatty acids from sea water.

Osterroht, Christoph (1993) Extraction of dissolved fatty acids from sea water. Fresenius' Journal of Analytical Chemistry, 345 (12). pp. 773-779. DOI 10.1007/BF00323009.

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Abstract

During a cruise in the Baltic Sea in March/April 1991, dissolved lipids were concentrated from acidified sea water by adsorption on reversed phase material; the free fatty acids in the lipids were isolated. Titration of the acids with 0.01 mol/l methanolic KOH resulted in a very poor titration curve; assessing total concentration of organic acids in sea water by base titration appears to be impractical. After derivatization with p-phenylphenacyl bromide under crown ether catalysis the fatty acids were analyzed by micro-bore HPLC with UV-detection and comparison with standard reference substances. Some unknown fatty acids were characterized by GC/MS. Total concentrations were in the range from 1 to 4.5 nmol/l. They were the same in parallel samples liquid/liquid extracted with dichloromethane. The HPLC spectra of the fatty acids in both kinds of samples were also quite similar. This confirms earlier observations that adsorptive concentration of dissolved fatty acids and non-polar lipids from sea water is as effective as liquid/liquid extraction. Higher amounts of fatty acids can be concentrated by adsorption from large water volumes, thus lowering detection limits. Palmitic acid had by far the highest concentration in every sample followed by myristic acid, lauric acid, and stearic acid. Palmitoleic acid and oleic acid were the most abundant unsaturated acids. The other saturated and unsaturated acids had medium to low concentrations.

Document Type: Article
Keywords: Oleic Acid; Stearic Acid; Palmitic Acid; Crown Ether; Lauric Acid
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-CH Chemical Oceanography
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1007/BF00323009
ISSN: 0937-0633
Date Deposited: 23 Mar 2018 11:15
Last Modified: 23 Mar 2018 11:15
URI: http://oceanrep.geomar.de/id/eprint/42381

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