Linear Aminolipids with Moderate Antimicrobial Activity from the Antarctic Gram-Negative Bacterium Aequorivita sp..

Chianese, Giuseppina, Esposito, Fortunato, Parrot, Delphine, Ingham, Colin, de Pascale, Donatella and Tasdemir, Deniz (2018) Linear Aminolipids with Moderate Antimicrobial Activity from the Antarctic Gram-Negative Bacterium Aequorivita sp.. Open Access Marine Drugs, 16 (6, Article Nr. 187). DOI 10.3390/md16060187.

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Abstract

The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of three new (1–3) and four known (4–7) N-terminal glycine- or serine-bearing iso-fatty acid amides esterified with another iso-fatty acid through their C-3 hydroxy groups. The chemical structures of the new compounds were elucidated using a set of spectroscopic (NMR, [α]D and FT-IR) and spectrometric (HRMS, HRMS/MS) methods. The aminolipids possessing an N-terminal glycine unit (1, 2, 4, 5) showed moderate in vitro antimicrobial activity against MRSA (IC50 values 22–145 µg/mL). This is the first in-depth chemistry and biological activity study performed on the microbial genus Aequorivita.

Document Type: Article
Keywords: Aequorivita; Gram-negative bacterium; miniaturized culture chip; linear aminolipid; MRSA; LC-MS/MS
Research affiliation: OceanRep > GEOMAR > Applied R&D > GEOMAR-Biotech
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.3390/md16060187
ISSN: 1660-3397
Date Deposited: 18 Jun 2018 12:58
Last Modified: 24 May 2019 12:39
URI: http://oceanrep.geomar.de/id/eprint/43438

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