In situ chemistry and microbial community compositions in five deep-sea hydrothermal fluid samples from Irina II in the Logatchev field.

Perner, Mirjam , Gonella, Giorgio, Hourdez, Stephane, Bohnke, Stefanie, Kurtz, Stefan and Girguis, Peter (2013) In situ chemistry and microbial community compositions in five deep-sea hydrothermal fluid samples from Irina II in the Logatchev field. Environmental Microbiology, 15 (5). pp. 1551-1560. DOI 10.1111/1462-2920.12038.

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Abstract

We present data on the co-registered geochemistry (in situ mass spectrometry) and microbiology (pyrosequencing of 16S rRNA genes; V1, V2, V3 regions) in five fluid samples from Irina II in the Logatchev hydrothermal field. Two samples were collected over 24min from the same spot and further three samples were from spatially distinct locations (20cm, 3m and the overlaying plume). Four low-temperature hydrothermal fluids from the Irina II are composed of the same core bacterial community, namely specific Gammaproteobacteria and Epsilonproteobacteria, which, however, differs in the relative abundance. The microbial composition of the fifth sample (plume) is considerably different. Although a significant correlation between sulfide enrichment and proportions of Sulfurovum (Epsilonproteobacteria) was found, no other significant linkages between abiotic factors, i.e. temperature, hydrogen, methane, sulfide and oxygen, and bacterial lineages were evident. Intriguingly, bacterial community compositions of some time series samples from the same spot were significantly more similar to a sample collected 20cm away than to each other. Although this finding is based on three single samples only, it provides first hints that single hydrothermal fluid samples collected on a small spatial scale may also reflect unrecognized temporal variability. However, further studies are required to support this hypothesis.

Document Type: Article
Additional Information: The sequence data have been deposited in the National Center for Biotechnology Information Sequence Read Archive (SRA) under the Accession Number SRP016119.
Keywords: MID-ATLANTIC RIDGE; AMPLICON PYROSEQUENCING BTEFAP; GEOCHEMICAL CONSTRAINTS; EPSILON-PROTEOBACTERIA; BACTERIAL DIVERSITY; RARE BIOSPHERE; VENT; VARIABILITY; SEQUENCES; HABITATS
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1111/1462-2920.12038
ISSN: 1462-2920
Expeditions/Models/Experiments:
Date Deposited: 18 Jul 2018 09:54
Last Modified: 18 Jul 2018 09:54
URI: http://oceanrep.geomar.de/id/eprint/43737

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