Whole-transcriptome analysis reveals genetic factors underlying flowering time regulation in rapeseed (Brassica napus L.).

Shah, Smit, Weinholdt, Claus, Jedrusik, Nicole, Molina, Carlos, Zou, Jun, Große, Ivo, Schiessl, Sarah, Jung, Christian and Emrani, Nazgol (2018) Whole-transcriptome analysis reveals genetic factors underlying flowering time regulation in rapeseed (Brassica napus L.). Plant Cell and Environment, 41 (8). pp. 1935-1947. DOI 10.1111/pce.13353.

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Supplementary data:


Rapeseed (Brassica napus L.), one of the most important sources of vegetable oil and protein‐rich meals worldwide, is adapted to different geographical regions by modification of flowering time. Rapeseed cultivars have different day length and vernalization requirements, which categorize them into winter, spring, and semiwinter ecotypes. To gain a deeper insight into genetic factors controlling floral transition in B. napus, we performed RNA sequencing (RNA‐seq) in the semiwinter doubled haploid line, Ningyou7, at different developmental stages and temperature regimes. The expression profiles of more than 54,000 gene models were compared between different treatments and developmental stages, and the differentially expressed genes were considered as targets for association analysis and genetic mapping to confirm their role in floral transition. Consequently, 36 genes with association to flowering time, seed yield, or both were identified. We found novel indications for neofunctionalization in homologs of known flowering time regulators like VIN3 and FUL. Our study proved the potential of RNA‐seq along with association analysis and genetic mapping to identify candidate genes for floral transition in rapeseed. The candidate genes identified in this study could be subjected to genetic modification or targeted mutagenesis and genotype building to breed rapeseed adapted to certain environments.

Document Type: Article
Keywords: association analysis, differentially expressed genes, genetic mapping, pleiotropic effects, RNA‐seq, vernalization, yield
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1111/pce.13353
ISSN: 0140-7791
Projects: Future Ocean
Date Deposited: 01 Aug 2018 09:37
Last Modified: 08 Apr 2019 09:21
URI: http://oceanrep.geomar.de/id/eprint/43889

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