EMS-induced point mutations in ALCATRAZ homoeologs increase silique shatter resistance of oilseed rape (Brassica napus).

Braatz, Janina, Harloff, Hans-Joachim and Jung, Christian (2018) EMS-induced point mutations in ALCATRAZ homoeologs increase silique shatter resistance of oilseed rape (Brassica napus). Open Access Euphytica, 214 (2). DOI 10.1007/s10681-018-2113-7.

Full text not available from this repository.

Supplementary data:

Abstract

In Arabidopsis (Arabidopsis thaliana), the transcription factor ALCATRAZ (ALC) is involved in the control of silique tissue identity, ensuring the establishment of a separation layer that contributes to the fragility of the dry fruit. The silique structure is retained in the related crop species oilseed rape (Brassica napus), in which it causes yield losses due to seed shedding. A more robust rapeseed silique through Bnalc loss-of-function mutations was hypothesized. We demonstrated the increased silique shatter resistance of oilseed rape through Cas9-induced targeted Bnalc mutations in a previous work. However, the effect was masked by the high shatter resistance of the transformed cultivar itself. In the present study, we used a rapeseed genotype with low shattering resistance and followed an approach of random mutagenesis. We identified 23 Bnalc mutants by TILLING of an EMS-mutagenized ‘Express’ population. By measuring tensile forces necessary to disrupt mature siliques, we determined a double mutant with significantly increased shatter resistance. This mutant can readily be introduced into breeding programs.

Document Type: Article
Keywords: Brassica napus, Canola, Dehiscence Zone, Mutants, Rapeseed, Siliques, TILLING
Research affiliation: Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1007/s10681-018-2113-7
ISSN: 0014-2336
Projects: Future Ocean
Date Deposited: 01 Aug 2018 09:38
Last Modified: 23 Sep 2019 23:44
URI: http://oceanrep.geomar.de/id/eprint/43890

Actions (login required)

View Item View Item