Porcine RPE/Choroidal Explant Cultures.

Klettner, Alexa Karina, Miura, Yoko, Langmann, Thomas and Weber, Bernhard H. F. (2018) Porcine RPE/Choroidal Explant Cultures. Retinal Degeneration, 1834 . pp. 109-118. DOI 10.1007/978-1-4939-8669-9_8.

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Abstract

The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

Document Type: Article
Keywords: Bruch’s membrane; Perfusion culture; Retinal pigment epithelium
Research affiliation: KIT
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1007/978-1-4939-8669-9_8
ISSN: 1064-3745
Projects: Future Ocean
Date Deposited: 06 Dec 2018 10:41
Last Modified: 24 Sep 2019 00:08
URI: http://oceanrep.geomar.de/id/eprint/44858

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