Seasonal Variations in the Metabolome and Bioactivity Profile of Fucus vesiculosus Extracted by an Optimised, Pressurised Liquid Extraction Protocol.

Heavisides, Edwin, Rouger, Caroline , Reichel, Anna, Ulrich, Cornelia, Wenzel-Storjohann, Arlette, Sebens, Susanne and Tasdemir, Deniz (2018) Seasonal Variations in the Metabolome and Bioactivity Profile of Fucus vesiculosus Extracted by an Optimised, Pressurised Liquid Extraction Protocol. Open Access Marine Drugs, 16 (12). Art.Nr. 503. DOI 10.3390/md16120503.

[img]
Preview
Text
marinedrugs-16-00503.pdf - Published Version
Available under License Creative Commons: Attribution 4.0.

Download (1336Kb) | Preview

Supplementary data:

Abstract

The metabolism of seaweeds depends on environmental parameters, the availability of nutrients, and biotic/abiotic stresses; therefore, their chemical composition fluctuates throughout the year. This study investigated seasonal variations in the metabolome of the Baltic Sea brown alga Fucus vesiculosus and its potential relation to the bioactivity profile. By using a definitive screening design (DSD) combined with pressurised liquid extraction (PLE), an optimised protocol was developed to extract algal biomass monthly for a full calendar year. An untargeted metabolomics approach using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MSn)-based molecular networking and manual dereplication was employed. The extracts were simultaneously screened for their in vitro antimicrobial, anticancer/apoptotic, and free radical scavenging activities. 44 compounds were putatively dereplicated in the metabolome. Many compounds were found to vary with the sampling month; phlorotannin total ion count (TIC) was highest in summer, whilst chlorophylls, lipids, and carotenoids peaked in winter and spring. The greatest radical scavenging and apoptotic activities against pancreas cancer cells observed in the summer months were attributed to high phlorotannin TIC. Methicillin-resistant Staphylococcus aureus (MRSA) inhibitory activity was produced year-round without a clear seasonal trend. This is the first study applying DSD-based optimised PLE extraction combined with a metabolome analysis of F. vesiculosus for the identification of seasonal variations in both metabolome and bioactivity.

Document Type: Article
Keywords: Fucus vesiculosus; bladder wrack; pressurised liquid extraction; definitive screening design; macroalga; untargeted metabolomics; seasonal variation; bioactivity
Research affiliation: OceanRep > GEOMAR > Applied R&D > GEOMAR-Biotech
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.3390/md16120503
ISSN: 1660-3397
Date Deposited: 07 Jan 2019 13:10
Last Modified: 24 May 2019 12:41
URI: http://oceanrep.geomar.de/id/eprint/45122

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...