Zinc Oxide Tetrapods Based Biohybrid Interface for Voltammetric Sensing of Helicobacter pylori.

Chauhan, Nidhi, Gupta, Shaivya, Avasthi, Devesh K., Adelung, Rainer, Mishra, Yogendra Kumar and Jain, Utkarsh (2018) Zinc Oxide Tetrapods Based Biohybrid Interface for Voltammetric Sensing of Helicobacter pylori. Open Access ACS Applied Materials & Interfaces, 10 (36). pp. 30631-30639. DOI 10.1021/acsami.8b08901.

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Supplementary data:

Abstract

Helicobacter pylori is a Gram-negative, spiral shaped, microaerophilic bacteria that colonizes human gastric mucosa and causes various gastric diseases. In this work, the utilization of ion irradiated zinc oxide tetrapods (ZnO-T) based biohybrid interface accentuates the development of an electrochemical immunosensor for the fast and sensitive detection of H. pylori. After coating of (ZnO-T) over the surface of screen printed electrode (SP-AuE) through electrodeposition, the ZnO-T/SP-AuE was irradiated with N2+ ion of energy 100 keV. The ion irradiation significantly enhances the conductivity of ZnO-T coated SP-AuE. The revamped SP-AuE is further used for establishing an immunosensor interface based upon immobilization of the CagA antigen on ZnO-T electrodeposited over the surface of SP-AuE. The sensing interface demonstrated good linearity (0.2 ng/mL to 50 ng/mL) and limit of detection (0.2 ng/mL). The ion beam irradiated ZnO-T based immunosensor showed significantly high conductivity and enhanced the analytical properties of the working electrode in terms of the sensitivity, detection limit, and response time. A study on the comparison of irradiated and pristine electrode is performed for amperometric sensing of H. pylori. In addition, the significance of work conducted on ion irradiated ZnO-T based interfaces provides a basis of further development of electrochemical immunosensors.

Document Type: Article
Keywords: CagA antigen; H. pylori; immunosensor; ion beam modification; screen printed electrode; zinc oxide tetrapods
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1021/acsami.8b08901
ISSN: 1944-8244
Projects: Future Ocean
Date Deposited: 16 Jan 2019 09:23
Last Modified: 27 Mar 2019 08:58
URI: http://oceanrep.geomar.de/id/eprint/45363

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