Resveratrol Modulates Desaturase Expression and Fatty Acid Composition of Cultured Hepatocytes.

Kühn, Gianna, Pallauf, Kathrin, Schulz, Carsten, Birringer, Marc, Diaz-Rica, Beatriz, de Pascual-Teresa, Sonia and Rimbach, Gerald (2018) Resveratrol Modulates Desaturase Expression and Fatty Acid Composition of Cultured Hepatocytes. Open Access Frontiers in Nutrition, 5 . DOI 10.3389/fnut.2018.00106.

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Abstract

This study aimed to evaluate whether resveratrol (RSV) and its microbial metabolites
dihydro-resveratrol (DHR) and lunularin (LUN) affected fatty acid metabolism and
omega-3 polyunsaturated fatty acid (n3-PUFA) synthesis in cultured hepatocytes. To this
end, cultured human HepG2 hepatocytes were treated with non-toxic concentrations
of these polyphenols (40μM) and �5- and �6-desaturase (FADS1 and FADS2,
respectively) expression was measured. Resveratrol induced both genes but DHR and
LUN showed no effect. Co-incubation of RSV with α-linolenic acid (ALA) also induced
FADS1 and FADS2 expression. Moreover, transcription of carnitine palmitoyltransferase
1A and fatty acid synthase expression was increased, indicating induction of β-oxidation
and fatty acid synthesis, respectively. Using gas chromatography to measure fatty acid
levels, we observed the impact of RSV with and without ALA treatment on fatty acid
composition. However, RSV reduced unsaturated while increasing saturated fatty acid
levels. We found lower amounts of monounsaturated fatty acids (16:1n-7c, 18:1n-9c,
18:1n7c, and 20:1n-9) and n3-PUFA docosahexaenoic acid whereas unsaturated fatty
acid levels, especially of stearic acid, were elevated. Of interest, once we co-incubated
the cells with RSV together with bovine serum albumin, we found no differences in gene
expression compared to cells without RSV treatment. Although we found no positive
effect of RSV on n3-PUFA synthesis, the stilbene could possibly prevent cellular stress
by decreasing unsaturated fatty acid levels.

Document Type: Article
Keywords: resveratrol, HepG2 cells, omega-3, desaturase, stearic acid
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.3389/fnut.2018.00106
ISSN: 2296-861X
Projects: Future Ocean
Date Deposited: 30 Jan 2019 12:07
Last Modified: 14 Mar 2019 11:37
URI: http://oceanrep.geomar.de/id/eprint/45589

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