In-vivo turnover frequency of the cyanobacterial NiFe-hydrogenase during photohydrogen production outperforms in-vitro systems.

Gutekunst, Kirstin, Hoffmann, Dörte, Westernströer, Ulrike, Schulz, Rüdiger, Garbe-Schönberg, Dieter and Appel, Jens (2018) In-vivo turnover frequency of the cyanobacterial NiFe-hydrogenase during photohydrogen production outperforms in-vitro systems. Open Access Scientific Reports, 8 (1). DOI 10.1038/s41598-018-24430-y.

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Supplementary data:

Abstract

Cyanobacteria provide all components for sunlight driven biohydrogen production. Their bidirectional NiFe-hydrogenase is resistant against low levels of oxygen with a preference for hydrogen evolution. However, until now it was unclear if its catalytic efficiency can keep pace with the photosynthetic electron transfer rate. We identified NikKLMQO (sll0381-sll0385) as a nickel transporter, which is required for hydrogen production. ICP-MS measurements were used to quantify hydrogenase molecules per cell. We found 400 to 2000 hydrogenase molecules per cell depending on the conditions. In-vivo turnover frequencies of the enzyme ranged from 62 H2/s in the wild type to 120 H2/s in a mutant during photohydrogen production. These frequencies are above maximum in-vivo photosynthetic electron transfer rates of 47 e−/s (equivalent to 24 H2/s). They are also above those of existing in-vitro systems working with unlimited electron supply and show that in-vivo photohydrogen production is limited by electron delivery to the enzyme.

Document Type: Article
Keywords: Mass spectrometry, Oxidoreductases
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1038/s41598-018-24430-y
ISSN: 2045-2322
Projects: Future Ocean
Date Deposited: 30 Jan 2019 12:15
Last Modified: 13 Mar 2019 10:07
URI: http://oceanrep.geomar.de/id/eprint/45593

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