3D Hydrogels Containing Interconnected Microchannels of Subcellular Size for Capturing Human Pathogenic Acanthamoeba Castellanii.

Gutekunst, Sören B., Siemsen, Katharina, Huth, Steven, Möhring, Anneke, Hesseler, Britta, Timmermann, Michael, Paulowicz, Ingo, Mishra, Yogendra Kumar, Siebert, Leonard, Adelung, Rainer and Selhuber-Unkel, Christine (2019) 3D Hydrogels Containing Interconnected Microchannels of Subcellular Size for Capturing Human Pathogenic Acanthamoeba Castellanii. ACS Biomaterials Science & Engineering, 5 (4). pp. 1784-1792. DOI 10.1021/acsbiomaterials.8b01009.

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Supplementary data:

Abstract

Porous hydrogel scaffolds are ideal candidates for mimicking cellular microenvironments, regarding both structural and mechanical aspects. We present a novel strategy to use uniquely designed ceramic networks as templates for generating hydrogels with a network of interconnected pores in the form of microchannels. The advantages of this new approach are the high and guaranteed interconnectivity of the microchannels, as well as the possibility to produce channels with diameters smaller than 7 μm. Neither of these assets can be ensured with other established techniques. Experiments using the polyacrylamide substrates produced with our approach have shown that the migration of human pathogenic Acanthamoeba castellanii trophozoites is manipulated by the microchannel structure in the hydrogels. The parasites can even be captured inside the microchannel network and removed from their incubation medium by the porous polyacrylamide, indicating the huge potential of our new technique for medical, pharmaceutical, and tissue engineering applications.

Document Type: Article
Keywords: 3D ceramic templates, microchannels, porous Hydrogel, cell migration
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1021/acsbiomaterials.8b01009
ISSN: 2373-9878
Projects: Future Ocean
Date Deposited: 01 Aug 2019 10:08
Last Modified: 02 Jan 2020 12:15
URI: http://oceanrep.geomar.de/id/eprint/47299

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