Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae.

Mittermayer, Felix H. , Stiasny, Martina H. , Clemmesen, Catriona , Bayer, Till , Puvanendran, V., Chierici, M., Jentoft, S. and Reusch, Thorsten B. H. (2019) Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae. Open Access Scientific Reports, 9 (1). Art.Nr. 16908. DOI 10.1038/s41598-019-52628-1.

[img]
Preview
Text
s41598-019-52628-1.pdf - Published Version
Available under License Creative Commons: Attribution 4.0.

Download (1064Kb) | Preview
[img]
Preview
Text
41598_2019_52628_MOESM1_ESM.pdf - Supplemental Material
Available under License Creative Commons: Attribution 4.0.

Download (938Kb) | Preview

Supplementary data:

Abstract

Ocean acidification (OA), a direct consequence of increasing atmospheric CO2 concentration dissolving in ocean waters, is impacting many fish species. Little is known about the molecular mechanisms underlying the observed physiological impacts in fish. We used RNAseq to characterize the transcriptome of 3 different larval stages of Atlantic cod (Gadus morhua) exposed to simulated OA at levels (1179 µatm CO2) representing end-of-century predictions compared to controls (503 µatm CO2), which were shown to induce tissue damage and elevated mortality in G. morhua. Only few genes were differentially expressed in 6 and 13 days-post-hatching (dph) (3 and 16 genes, respectively), during a period when maximal mortality as a response to elevated pCO2 occurred. At 36 dph, 1413 genes were differentially expressed, most likely caused by developmental asynchrony between the treatment groups, with individuals under OA growing faster. A target gene analysis revealed only few genes of the universal and well-defined cellular stress response to be differentially expressed. We thus suggest that predicted ocean acidification levels constitute a “stealth stress” for early Atlantic cod larvae, with a rapid breakdown of cellular homeostasis leading to organismal death that was missed even with an 8-fold replication implemented in this study.

Document Type: Article
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
Kiel University
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1038/s41598-019-52628-1
ISSN: 2045-2322
Related URLs:
Projects: BONUS BIO-C3, BIOACID
Date Deposited: 18 Nov 2019 08:48
Last Modified: 18 Nov 2019 08:48
URI: http://oceanrep.geomar.de/id/eprint/48244

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...