Differential gene expression patterns related to lipid metabolism in response to ocean acidification in larvae and juveniles of Atlantic cod.

Frommel, Andrea, Hermann, Bernd T., Michael, K., Lucassen, M., Clemmesen, Catriona , Hanel, Reinhold and Reusch, Thorsten B. H. (2020) Differential gene expression patterns related to lipid metabolism in response to ocean acidification in larvae and juveniles of Atlantic cod. Comparative Biochemistry and Physiology A - Molecular and Integrative Physiology, 247 . Art.Nr. 110740. DOI 10.1016/j.cbpa.2020.110740.

[img] Text
Frommel_Hermann_etal_2020.pdf - Published Version
Restricted to Registered users only

Download (663Kb) | Contact
[img]
Preview
Image (Graphical abstract)
1-s2.0-S1095643320300921-ga1_lrg.jpg - Supplemental Material
Available under License Creative Commons: Attribution 4.0.

Download (234Kb) | Preview

Supplementary data:

Abstract

Highlights:
• Larvae upregulate genes associated with fatty acid and glycogen synthesis under moderate ocean acidification (OA)
• Larvae under high levels of OA fail to regulate
• Dysfunctional metabolism and stress associated with pathologies in internal organs
• Lack of differential gene regulation and stress response in juveniles correspond to a higher resilience to OA stress

Elevated environmental carbon dioxide (pCO2) levels have been found to cause organ damage in the early life stages of different commercial fish species, including Atlantic cod (Gadus morhua). To illuminate the underlying mechanisms causing pathologies in the intestines, the kidney, the pancreas and the liver in response to elevated pCO2, we examined related gene expression patterns in Atlantic cod reared for two months under three different pCO2 regimes: 380 μatm (control), 1800 μatm (medium) and 4200 μatm (high). We extracted RNA from whole fish sampled during the larval (32 dph) and early juvenile stage (46 dph) for relative expression analysis of 18 different genes related to essential metabolic pathways. At 32 dph, larvae subjected to the medium treatment displayed an up-regulation of genes mainly associated with fatty acid and glycogen synthesis (GYS2, 6PGL, ACoA, CPTA1, FAS and PPAR1b). Larvae exposed to the high pCO2 treatment upregulated fewer but similar genes (6PGL, ACoA and PPAR1b,). These data suggest stress-induced alterations in the lipid and fatty acid metabolism and a disrupted lipid homeostasis in larvae, providing a mechanistic link to the findings of lipid droplet overload in the liver and organ pathologies. At 46 dph, no significant differences in gene expression were detected, confirming a higher resilience of juveniles in comparison to larvae when exposed to elevated pCO2 up to 4200 μatm.

Document Type: Article
Funder compliance: info:eu-repo/grantAgreement/EC/FP7/211384
Dewey Decimal Classification: 500 Natural Sciences and Mathematics > 570 Life sciences; biology
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
AWI
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.cbpa.2020.110740
ISSN: 1095-6433
Projects: ISOS, EPOCA, BIOACID
Date Deposited: 08 Jun 2020 09:03
Last Modified: 08 Jun 2020 09:18
URI: http://oceanrep.geomar.de/id/eprint/49829

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...