Simultaneous δ15N, δ13C and δ34S measurements of low-biomass samples using a technically advanced high sensitivity elemental analyzer connected to an isoptope ratio mass spectrometer.

Hansen, Thomas, Burmeister, Antje and Sommer, Ulrich (2009) Simultaneous δ15N, δ13C and δ34S measurements of low-biomass samples using a technically advanced high sensitivity elemental analyzer connected to an isoptope ratio mass spectrometer. Rapid Communications in Mass Spectrometry, 23 (21). pp. 3387-3393. DOI 10.1002/rcm.4267.

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Abstract

Conventional simultaneous CNS stable isotope abundance measurements of solid samples usually require high sample amounts, up to 1 mg carbon, to achieve exact analytical results. This rarely used application is often impaired by high C:S element ratios when organic samples are analyzed and problems such as incomplete conversion into sulphur dioxide occur during analysis. We introduce, as a technical innovation, a high sensitivity elemental analyzer coupled to a conventional isotope ratio mass spectrometer, with which CNS-stable isotope ratios can be determined simultaneously in samples with low carbon content (<40 mu g C corresponding to similar to 100 mu g dry weight). The system includes downsized reactors, a temperature program-controlled gas chromatography (GC) column and a cryogenic trap to collect small amounts of sulphur dioxide. This modified application allows for highly sensitive measurements in a fully automated operation with standard deviations better than +/-0.47 parts per thousand for delta N-15 and delta S-34 and +/-0.12 parts per thousand for delta C-13 (n=127). Samples collected from one sampling site in a Baltic fjord within a short time period were measured with the new system to get a first impression of triple stable isotope signatures. The results confirm the potential of using delta S-34 as a stable isotope tracer in combination with delta N-15 and delta C-13 measurements to improve discrimination of food sources in aquatic food webs. Copyright (C) 2009 John Wiley & Sons, Ltd

Document Type: Article
Keywords: Food Webs; food-web analysisstable-isotopes sulfur nitrogen sulfate carbon fractionation temperature delta-o-18 conversion
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-N Experimental Ecology - Food Webs
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1002/rcm.4267
ISSN: 0951-4198
Projects: Future Ocean
Date Deposited: 10 Nov 2009 13:31
Last Modified: 15 Sep 2016 09:40
URI: http://oceanrep.geomar.de/id/eprint/6288

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