Diel rhythm of nitrogen and carbon metabolism in the unicellular, diazotrophic cyanobacterium Crocosphaera watsonii WH8501.

Mohr, Wiebke, Intermaggio, M.P. and LaRoche, Julie (2010) Diel rhythm of nitrogen and carbon metabolism in the unicellular, diazotrophic cyanobacterium Crocosphaera watsonii WH8501. Environmental Microbiology, 12 (2). pp. 412-421. DOI 10.1111/j.1462-2920.2009.02078.x.

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Abstract

We examined the diel variation in nitrogen and carbon metabolism in Crocosphaera watsonii WH8501 at the physiological and gene expression level in order to determine the temporal constraints for N2 fixation and photosynthesis. N2 fixation and photosynthesis were restricted to the dark and light periods, respectively, during a 24 h light–dark cycle. All genes studied here except one (psbA2) showed diel variations in their expression levels. The highest variation was seen in nifH and nifX relative transcript abundance with a factor of 3–5 × 103 between light and dark periods. Photosynthesis genes showed less variation with a maximum factor of about 500 and always had high relative transcript abundances relative to other genes. At the protein level, the photosystems appeared more stable than the nitrogenase complex over a 24 h light–dark cycle, suggesting that C. watsonii retains the ability to photosynthesize during the dark period of the diel cycle. In contrast, nitrogenase is synthesized daily and exhibits peak abundance during the dark period. Our results have implications for field studies with respect to the interpretation of environmental gene expression data.

Document Type: Article
Keywords: Marine Biology; Cyanobacteria; Crocosphaera watsonii; NORTH PACIFIC-OCEAN; LIGHT-GROWN CULTURES; SP STRAIN ATCC-51142; SP IMS 101; ATCC 51142; TRANSLATIONAL REGULATION; FIXING CYANOBACTERIA; CIRCADIAN-RHYTHM; GENE-EXPRESSION; ATLANTIC-OCEAN
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1111/j.1462-2920.2009.02078.x
ISSN: 1462-2920
Projects: Future Ocean, SOPRAN
Date Deposited: 20 Oct 2009 09:43
Last Modified: 12 Sep 2018 12:48
URI: http://oceanrep.geomar.de/id/eprint/7497

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