The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla).

Marohn, Lasse, Rehbein, H., Kündiger, R. and Hanel, Reinhold (2008) The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla). Journal of Biotechnology, 136 . pp. 135-139. DOI 10.1016/j.jbiotec.2008.06.008.

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Abstract

European eel (Anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3′,4,4′-tetrachlorobiyphenyl (PCB77) to examine and characterize the inductive effect of coplanar PCBs on CYP1A1 gene expression in liver and gills by using a semi-quantitative RT-PCR analysis. The influence of PCB77 injection on transcription activity of the housekeeping gene glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) was tested to determine its suitability as a reference gene for further quantitative gene expression analyses. Our results clearly indicate a significant dose-dependent increase in CYP1A1 gene expression in the gills of European eel, while in liver tissues a significant elevation in CYP1A1 gene expression was only detectable at highest contamination rates, indicating the potential of CYP1A1 differential gene expression analysis in gills as a biomarker for PCB contamination in eels. PCB77 contamination did not affect GAPDH transcription in gills but, at highest doses, resulted in a significant elevation in liver, speaking against GAPDH as a reference housekeeping gene after PCB exposure.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.jbiotec.2008.06.008
ISSN: 0168-1656
Projects: Habitat Selection of the European Eel
Date Deposited: 24 Apr 2009 11:15
Last Modified: 02 Oct 2018 09:11
URI: http://oceanrep.geomar.de/id/eprint/7653

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