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The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla).
Marohn, Lasse, Rehbein, H., Kündiger, R. and Hanel, Reinhold (2008) The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla). Journal of Biotechnology, 136 . pp. 135-139. DOI 10.1016/j.jbiotec.2008.06.008.
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Abstract
European eel (Anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3′,4,4′-tetrachlorobiyphenyl (PCB77) to examine and characterize the inductive effect of coplanar PCBs on CYP1A1 gene expression in liver and gills by using a semi-quantitative RT-PCR analysis. The influence of PCB77 injection on transcription activity of the housekeeping gene glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) was tested to determine its suitability as a reference gene for further quantitative gene expression analyses. Our results clearly indicate a significant dose-dependent increase in CYP1A1 gene expression in the gills of European eel, while in liver tissues a significant elevation in CYP1A1 gene expression was only detectable at highest contamination rates, indicating the potential of CYP1A1 differential gene expression analysis in gills as a biomarker for PCB contamination in eels. PCB77 contamination did not affect GAPDH transcription in gills but, at highest doses, resulted in a significant elevation in liver, speaking against GAPDH as a reference housekeeping gene after PCB exposure.
Document Type: | Article |
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Research affiliation: | OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology |
Refereed: | Yes |
Open Access Journal?: | No |
Publisher: | Elsevier |
Projects: | Habitat Selection of the European Eel |
Date Deposited: | 24 Apr 2009 11:15 |
Last Modified: | 02 Oct 2018 09:11 |
URI: | https://oceanrep.geomar.de/id/eprint/7653 |
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