The effect of quorum-sensing blockers on the formation of marine microbial communities and larval attachment.

Dobretsov, Sergey V., Dahms, H.-U., Huang, Y. L., Wahl, Martin and Qian, P.-Y. (2007) The effect of quorum-sensing blockers on the formation of marine microbial communities and larval attachment. FEMS Microbiology Ecology, 60 (2). pp. 177-188. DOI 10.1111/j.1574-6941.2007.00285.x .

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Abstract

We studied the effect of the quorum-sensing (QS) blockers 5-hydroxy-3[(1R)-1-hydroxypropyl]-4-methylfuran-2(5H)-one (FUR1), (5R)-3,4-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]furan-2(5H)-one (FUR2) and triclosan (TRI) on the formation of bacterial biofilms, and the effect of these biofilms on the larval attachment of the polychaete Hydroides elegans and the bryozoan Bugula neritina. 14-day-old subtidal biofilms were harvested from artificial substrata and were allowed to develop in the laboratory with and without QS blockers. QS blockers inhibited the production of violacein by the QS reporter strain Chromobacterium violaceum CV026 and did not affect the metabolic activity of bacteria in multispecies biofilms. At a concentration of 10(-3) M all three tested compounds inhibited the establishment of microbial communities, but at one of 10(-4) M only FUR2 inhibited establishment. The tested QS blockers caused changes in bacterial density and bacterial community structure, as revealed by terminal restriction fragment length polymorphism and FISH. The groups most affected by QS blockers were Alphaproteobacteria, Gammaproteobacteria and the Cytophagales. Larvae of H. elegans and B. neritina avoided settling on biofilms that had developed in the presence of QS blockers. Our results suggest that QS blockers directly control the formation of multi-species biofilms, and indirectly - by means of biofilm properties - affect larval attachment on these modified biofilms.

Document Type: Article
Keywords: microbial communities; quorum-sensing inhibitors; larval attachment; furanones; antifouling
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1111/j.1574-6941.2007.00285.x
ISSN: 0168-6496
Projects: Future Ocean
Date Deposited: 03 Dec 2008 16:51
Last Modified: 05 Jan 2017 09:18
URI: http://oceanrep.geomar.de/id/eprint/7715

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