Comprehensive Investigation of Marine Actinobacteria Associated with the Sponge Halichondria panicea.

Schneemann, Imke, Nagel, Kerstin, Kajahn, Inga, Labes, Antje, Wiese, Jutta and Imhoff, Johannes F. (2010) Comprehensive Investigation of Marine Actinobacteria Associated with the Sponge Halichondria panicea. Applied and Environmental Microbiology, 76 (11). pp. 3702-3714. DOI 10.1128/AEM.00780-10.

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Abstract

Representatives of Actinobacteria were isolated from the marine sponge Halichondria panicea collected from the Baltic Sea (Germany). For the first time, a comprehensive investigation was performed with regard to phylogenetic strain identification, secondary metabolite profiling, bioactivity determination, and genetic exploration of biosynthetic genes, especially concerning the relationships of the abundance of biosynthesis gene fragments to the number and diversity of produced secondary metabolites. All strains were phylogenetically identified by 16S rRNA gene sequence analyses and were found to belong to the genera Actinoalloteichus, Micrococcus, Micromonospora, Nocardiopsis, and Streptomyces. Secondary metabolite profiles of 46 actinobacterial strains were evaluated, 122 different substances were identified, and 88 so far unidentified compounds were detected. The extracts from most of the cultures showed biological activities. In addition, the presence of biosynthesis genes encoding polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) in 30 strains was established. It was shown that strains in which either PKS or NRPS genes were identified produced a significantly higher number of metabolites and exhibited a larger number of unidentified, possibly new metabolites than other strains. Therefore, the presence of PKS and NRPS genes is a good indicator for the selection of strains to isolate new natural products.

Document Type: Article
Additional Information: WOS:000277871400034
Keywords: Microbiology; Biology; BIOSYNTHESIS GENE-CLUSTER; COMPLETE GENOME SEQUENCE; STREPTOMYCES SP; BIOLOGICAL PROPERTIES; POLYKETIDE SYNTHASE; NATURAL-PRODUCTS; PSEUDOCERATINA-CLAVATA; ANTIMYCIN ANTIBIOTICS; STRUCTURE ELUCIDATION; CHONDRILLA-NUCULA
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > GEOMAR > Applied R&D > Centre for Marine Substances (KiWiZ)
Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1128/AEM.00780-10
ISSN: 0099-2240
Projects: KIWIZ, Future Ocean
Date Deposited: 08 Jun 2010 12:03
Last Modified: 26 Apr 2016 12:20
URI: http://oceanrep.geomar.de/id/eprint/8488

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