Construction and Screening of Marine Metagenomic Libraries .

Weiland-Bräuer, Nancy, Löscher, Carolin, Metzger, Rebekka and Schmitz, Ruth A. (2010) Construction and Screening of Marine Metagenomic Libraries . In: Metagenomics: Methods and Protocols. , ed. by Streit, Wolfgang R. and Daniel, Rolf. Methods in Molecular Biology , 668 . Humana Press, New York, NJ, pp. 51-65. ISBN 978-1-607-61822-5 DOI 10.1007/978-1-60761-823-2_3.

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Abstract

Marine microbial communities are highly diverse and have evolved during extended evolutionary processes of physiological adaptations under the influence of a variety of ecological conditions and selection pressures. They harbor an enormous diversity of microbes with still unknown and probably new physiological characteristics. Besides, the surfaces of marine multicellular organisms are typically covered by a consortium of epibiotic bacteria and act as barriers, where diverse interactions between microorganisms and hosts take place. Thus, microbial diversity in the water column of the oceans and the microbial consortia on marine tissues of multicellular organisms are rich sources for isolating novel bioactive compounds and genes. Here we describe the sampling, construction of large-insert metagenomic libraries from marine habitats and exemplarily one function based screen of metagenomic clones.

Document Type: Book chapter
Keywords: Microbiology; Isolation of metagenomic DNA, 16S rDNA phylogenetic analysis, Fosmid library, Function-based screen
Research affiliation: OceanRep > SFB 754 > B4
OceanRep > SFB 754
Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > SFB 754 > B3
Open Access Journal?: No
DOI etc.: 10.1007/978-1-60761-823-2_3
Projects: SFB754
Date Deposited: 30 Aug 2011 10:00
Last Modified: 23 Sep 2019 19:34
URI: http://oceanrep.geomar.de/id/eprint/8561

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