Repeat induced point mutation in two asexual fungi, Aspergillus niger and Penicillium chrysogenum.

Braumann, I., van den Berg, M. and Kempken, Frank (2008) Repeat induced point mutation in two asexual fungi, Aspergillus niger and Penicillium chrysogenum. Current Genetics, 53 (5). pp. 287-297. DOI https://doi.org/10.1007/s00294-008-0185-y.

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Abstract

Repeat induced point mutation (RIP) is a gene silencing mechanism present in fungal genomes. During RIP, duplicated sequences are efficiently and irreversibly mutated by transitions from C:G to T:A. For the first time, we have identified traces of RIP in transposable elements of Aspergillus niger and Penicillium chrysogenum, two biotechnologically relevant fungi. We found that RIP in P. chrysogenum has affected a large set of sequences, which also contain other mutations. On the other hand, RIP in A. niger is limited to only few sequences, but literally all mutations are RIP-like. Surprisingly, RIP occurred only in transposon sequences that have disrupted open reading frames in A. niger, a phenomenon not yet reported for other fungi. In both fungal species, we identified two sequences with strong sequence similarity to Neurospora crassa RID. RID is a putative DNA methyltransferase and the only known enzyme involved in the RIP process. Our findings suggest that both A. niger and P. chrysogenum either had a sexual past or have a sexual potential. These findings have important implications for future strain development of these fungi.

Document Type: Article
Keywords: Repeat induced point mutation, Aspergillus niger, Penicillium chrysogenum, Transposons
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: https://doi.org/10.1007/s00294-008-0185-y
ISSN: 0172-8083
Projects: Future Ocean
Date Deposited: 15 Oct 2010 08:49
Last Modified: 26 Feb 2019 12:09
URI: http://oceanrep.geomar.de/id/eprint/8970

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