Methodological Underestimation of Oceanic Nitrogen Fixation Rates.

Mohr, Wiebke, Wallace, Douglas W.R., Großkopf, Tobias and LaRoche, Julie (2010) Methodological Underestimation of Oceanic Nitrogen Fixation Rates. Open Access PLoS ONE, 5 (9). e12583. DOI 10.1371/journal.pone.0012583.

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Abstract

The two commonly applied methods to assess dinitrogen (N2) fixation rates are the 15N2-tracer addition and the acetylene reduction assay (ARA). Discrepancies between the two methods as well as inconsistencies between N2 fixation rates and biomass/growth rates in culture experiments have been attributed to variable excretion of recently fixed N2. Here we demonstrate that the 15N2-tracer addition method underestimates N2 fixation rates significantly when the 15N2 tracer is introduced as a gas bubble. The injected 15N2 gas bubble does not attain equilibrium with the surrounding water leading to a 15N2 concentration lower than assumed by the method used to calculate 15N2-fixation rates. The resulting magnitude of underestimation varies with the incubation time, to a lesser extent on the amount of injected gas and is sensitive to the timing of the bubble injection relative to diel N2 fixation patterns. Here, we propose and test a modified 15N2 tracer method based on the addition of 15N2-enriched seawater that provides an instantaneous, constant enrichment and allows more accurate calculation of N2 fixation rates for both field and laboratory studies. We hypothesise that application of N2 fixation measurements using this modified method will significantly reduce the apparent imbalances in the oceanic fixed-nitrogen budget.

Document Type: Article
Keywords: Marine Biology; ocean nitrogen
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-CH Chemical Oceanography
OceanRep > SFB 754 > B3
OceanRep > SFB 754 > A3
OceanRep > SFB 754 > B4
OceanRep > SFB 754
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1371/journal.pone.0012583
ISSN: 1932-6203
Projects: SFB754
Expeditions/Models/Experiments:
Date Deposited: 01 Oct 2010 10:12
Last Modified: 21 Jan 2019 13:48
URI: http://oceanrep.geomar.de/id/eprint/9203

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