A German Chlamydia trachomatis screening program employing semi-automated real-time PCR: results and perspectives.

Bohm, I., Groning, A., Sommer, B., Muller, H. W., Krawczak, Michael and Glaubitz, R. (2009) A German Chlamydia trachomatis screening program employing semi-automated real-time PCR: results and perspectives. Journal of Clinical Virology, 46 . S27-S32. DOI 10.1016/S1386-6532(09)70298-7.

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Abstract

Background: Genital Chlamydia trachomatis infection is a worldwide public health burden. A screening program for C trachomatis was therefore initiated by the public health insurers in Germany ("Gemeinsamer Bundesausschuss", GBA) in April 2008.Objectives: To estimate C trachomatis prevalence from screening 115,766 asymptomatic females and 20,033 female patients with unspecific abdominal pain.
Study design: Urine samples (pooled by five for the asymptomatic screening subjects) and cervical swabs were analyzed using semi-automated real-time PCR. Infection prevalence was determined separately in four categories of women, defined by health status (asymptomatic screening vs. non-screening with unspecified symptoms) and test material used. Comparative analyses were stratified by age and pregnancy status.
Results: Experimental evaluation of the assay used revealed a detection limit of 379 genome copies/ml urine. For pooled urine samples, the positive predictive value was 100% whereas the negative predictive value equaled 98.1%. The observed infection prevalence was higher for cervical swabs than for urine samples. Prevalence estimates also differed significantly between pregnant and non-pregnant adolescents (<= 20 years), irrespective of the test material used (10.2% vs. 7.3% for cervical swabs, 10.9% vs. 6.1% for pooled urine samples).
Conclusions: Our retrospective study, based upon a very large number of females from all parts of Germany, revealed a high infection prevalence in adolescents, particularly in pregnant adsolescents, thereby justifying the screening directive of the German GBA. (C) 2009 Elsevier B.V. All rights reserved.

Document Type: Article
Additional Information: Suppl. 3528NZ
Keywords: chlamydia trachomatisscreening; real-time pcr pooling; public health; sexually-transmitted-diseases; ligase chain-reaction; neisseria-gonorrhoeae; cobas amplicor; urine specimens pregnant-women; united-kingdom; 1st-void urine vaginal swabs; prevalence;
Research affiliation: Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/S1386-6532(09)70298-7
ISSN: 1386-6532
Projects: Future Ocean
Date Deposited: 11 Feb 2011 12:16
Last Modified: 03 Jul 2017 12:18
URI: http://oceanrep.geomar.de/id/eprint/9304

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