RNA processing in plant mitochondria is independent of transcription.

Hinrichsen, I., Bolle, N., Paun, L. and Kempken, Frank (2009) RNA processing in plant mitochondria is independent of transcription. Plant Molecular Biology, 70 (6). pp. 663-668. DOI 10.1007/s11103-009-9498-6.

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Supplementary data:

Abstract

We analyzed the ability of plant mitochondria to process introduced RNA. Arabidopsis thaliana cox2 transcripts were synthesized in vitro. The in vitro transcribed mRNA was electroporated into maize and cauliflower mitochondria and incubated in organello. RNA was isolated and RT-PCR was carried out to analyze RNA processing. Our data indicate that cox2 transcripts introduced into isolated plant mitochondria are processed completely. This is the first report of in organello editing of introduced transcripts. We also found that none of the transcription, translation, or respiration inhibitors we used influenced RNA splicing or RNA editing of the cox2 transcript. Thus, our data also demonstrate that plant mitochondrial RNA processing may be independent of both transcription and respiratory regulation.

Document Type: Article
Keywords: in vitro cox2 mrnain organello rna editing rna splicing mitochondria arabidopsis-thaliana organello systems atp6 transcripts messenger-rnas translation electroporation 5'-end maize
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1007/s11103-009-9498-6
ISSN: 0167-4412
Projects: Future Ocean
Date Deposited: 11 Feb 2011 12:16
Last Modified: 19 Dec 2016 14:26
URI: http://oceanrep.geomar.de/id/eprint/9449

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