OceanRep
Conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and delta B-11.
Heinemann, Agnes, Fietzke, Jan , Melzner, Frank, Böhm, Florian, Thomsen, Jörn, Garbe-Schönberg, Dieter and Eisenhauer, Anton (2012) Conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and delta B-11. Geochemistry, Geophysics, Geosystems, 13 (1). Q01005. DOI 10.1029/2011GC003790.
Preview |
Text
ggge2075.pdf - Published Version Download (874kB) | Preview |
Abstract
Mytilus edulis were cultured for 3 months under six different seawater pCO(2) levels ranging from 380 to 4000 mu atm. Specimen were taken from Kiel Fjord (Western Baltic Sea, Germany) which is a habitat with high and variable seawater pCO(2) and related shifts in carbonate system speciation (e. g., low pH and low CaCO3 saturation state). Hemolymph (HL) and extrapallial fluid (EPF) samples were analyzed for pH and total dissolved inorganic carbon (C-T) to calculate pCO(2) and [HCO3-]. A second experiment was conducted for 2 months with three different pCO(2) levels (380, 1400 and 4000 mu atm). Boron isotopes (delta B-11) were investigated by LA-MC-ICP-MS (Laser Ablation-Multicollector-Inductively Coupled Plasma-Mass Spectrometry) in shell portions precipitated during experimental treatment time. Additionally, elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF of specimen from the second experiment were measured via ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). Extracellular pH was not significantly different in HL and EPF but systematically lower than ambient water pH. This is due to high extracellular pCO(2) values, a prerequisite for metabolic CO2 excretion. No accumulation of extracellular [HCO3-] was measured. Elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF increased slightly with pH which is in accordance with increasing growth and calcification rates at higher seawater pH values. Boron isotope ratios were highly variable between different individuals but also within single shells. This corresponds to a high individual variability in fluid B/Ca ratios and may be due to high boron concentrations in the organic parts of the shell. The mean delta B-11 value shows no trend with pH but appears to represent internal pH (EPF) rather than ambient water pH.
Document Type: | Article |
---|---|
Additional Information: | WOS:000299176800001 |
Keywords: | mytilus edulis; acid base; boron isotopes; extrapallial fluid; ocean acidification; trace metals; ocean acidification; calcification rate pecten-maximus; seawater acidification biological-controls; anthropogenic co2 |
Research affiliation: | OceanRep > The Future Ocean - Cluster of Excellence > FO-R09 Kiel University OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-MG Marine Geosystems OceanRep > The Future Ocean - Cluster of Excellence OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology |
Refereed: | Yes |
Open Access Journal?: | Yes |
Publisher: | AGU (American Geophysical Union) |
Projects: | Future Ocean, BIOACID |
Date Deposited: | 14 May 2014 10:03 |
Last Modified: | 23 Sep 2019 21:19 |
URI: | https://oceanrep.geomar.de/id/eprint/24033 |
Actions (login required)
View Item |
Copyright 2023 | GEOMAR Helmholtz-Zentrum für Ozeanforschung Kiel | All rights reserved
Questions, comments and suggestions regarding the GEOMAR repository are welcomed
at bibliotheksleitung@geomar.de !