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Fractionation, enzyme inhibitory and cellular antioxidant activity of bioactives from purple sweet potato ( Ipomoea batatas ).
Esatbeyoglu, Tuba, Rodríguez-Werner, Miriam, Schlösser, Anke, Winterhalter, Peter and Rimbach, Gerald (2017) Fractionation, enzyme inhibitory and cellular antioxidant activity of bioactives from purple sweet potato ( Ipomoea batatas ). Food Chemistry, 221 . pp. 447-456. DOI 10.1016/j.foodchem.2016.10.077.
Full text not available from this repository.Abstract
Sweet potato (Ipomoea batatas L.) is mainly cultivated in Asia. The deep purple color of purple sweet potato (PSP) is due to the high content of acylated anthocyanins. In the present study, PSP-derived polyphenols were identified using HPLC-PDA and HPLC-ESI-MSn analyses. After concentration of the polyphenols from PSP, preparative separation into two fractions, designated anthocyanins (AF) and copigments (CF), was carried out using adsorptive membrane chromatography. In enzyme inhibitory assays, all PSP samples inhibited the enzymes α-amylase, α-glucosidase and xanthine oxidase. Additionally, the cell signaling cellular antioxidant properties of the PSP extracts were investigated in cultured cells. PSP induced the transcription factor Nrf2, which regulates the expression of genes encoding heme oxygenase 1 (Hmox1), glutamate-cysteine ligase catalytic subunit (Gclc) and paraoxonase 1 (PON1). Furthermore, PSP enhanced cellular glutathione concentrations and decreased lipid peroxidation in cultured hepatocytes. Overall, these results suggest that PSP extracts exhibit enzyme inhibitory and cellular antioxidant properties, especially PSP CF.
Document Type: | Article |
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Keywords: | Anthocyanins; Cell culture; Chlorogenic acids; Enzyme inhibitory activity; HPLC; Membrane chromatography |
Research affiliation: | Kiel University Kiel University > Kiel Marine Science OceanRep > The Future Ocean - Cluster of Excellence |
Refereed: | Yes |
Open Access Journal?: | No |
Publisher: | Elsevier |
Projects: | Future Ocean |
Date Deposited: | 19 Dec 2017 15:39 |
Last Modified: | 23 Sep 2019 23:10 |
URI: | https://oceanrep.geomar.de/id/eprint/40954 |
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