Diversity of Bacteroidetes in high altitude saline evaporitic basins in northern Chile.

Dorador, Cristina, Meneses, D., Urtuvia, V., Demergasso, C., Vila, I., Witzel, K.-P. and Imhoff, Johannes F. (2009) Diversity of Bacteroidetes in high altitude saline evaporitic basins in northern Chile. Open Access Journal of Geophysical Research: Biogeosciences, 114 . G00D05. DOI 10.1029/2008JG000837.

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The phylum Bacteroidetes represents one of the most abundant bacterial groups of marine and freshwater bacterioplankton. We investigated the diversity of Bacteroidetes in water and sediment samples from three evaporitic basins located in the highlands of northern Chile. We used both 16S rRNA gene clone libraries created with targeted Bacteroidetes-specific primers and separation of specifically amplified gene fragments by denaturing gradient gel electrophoresis (DGGE). DGGE analysis revealed a reduced richness of these organisms in samples from Salar de Huasco (two to four DGGE bands) increasing in Salar de Ascotán (two to seven DGGE bands) and Laguna Tebenquiche at Salar de Atacama (four to eight DGGE bands). Cluster analysis (WPGMA) of DGGE bands showed that bands from Salar de Huasco and Salar de Ascotán grouped together and samples from Salar de Atacama formed separate clusters in water and sediment samples, reflecting different Bacteroidetes communities between sites. Most of the sequences analyzed belonged to the family Flavobacteriaceae and clustered with the genera Psychroflexus, Gillisia, Maribacter, Muricauda, Flavobacterium, and Salegentibacter. The most abundant phylotype was highly related to Psychroflexus spp. and was recovered from all three study sites. The similarity of the analyzed sequences with their closest relatives in GenBank was typically <97% and notably lower when compared with type strains, demonstrating the unique character of these sequences. Culture efforts will be necessary to get a better description of the diversity of this group in saline evaporitic basins of northern Chile.

Document Type: Article
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1029/2008JG000837
ISSN: 2169-8953
Projects: Future Ocean
Date Deposited: 12 Jan 2010 10:40
Last Modified: 23 Sep 2019 19:31
URI: http://oceanrep.geomar.de/id/eprint/1233

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