Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing.

Weiland-Bräuer, Nancy, Pinnow, Nicole and Schmitz, Ruth A. (2014) Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing. Applied and Environmental Microbiology, 81 (4). pp. 1477-1489. DOI 10.1128/aem.03290-14.

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Supplementary data:

Abstract

Two reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [ QS]). The basic design of these Escherichia coli-based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of QS signal molecules. Consequently, these E. coli strains are unable to grow in the presence of the respective QS signal molecules unless a nontoxic QS-interfering compound is present. The first reporter strain designed to detect autoinducer-2 (AI-2)-interfering activities (AI2-QQ. 1) contained the E. coli ccdB lethal gene under the control of the E. coli lsrA promoter. The second reporter strain (AI1-QQ. 1) contained the Vibrio fischeri luxI promoter fused to the ccdB gene to detect interference with acyl-homoserine lactones. Bacteria isolated from the surfaces of several marine eukarya were screened for quorum- quenching (QQ) activities using the established reporter systems AI1-QQ. 1 and AI2-QQ. 1. Out of 34 isolates, two interfered with acylated homoserine lactone (AHL) signaling, five interfered with AI-2 QS signaling, and 10 were demonstrated to interfere with both signal molecules. Open reading frames (ORFs) conferring QQ activity were identified for three selected isolates (Photobacterium sp., Pseudoalteromonas sp., and Vibrio parahaemolyticus). Evaluation of the respective heterologously expressed and purified QQ proteins confirmed their ability to interfere with the AHL and AI-2 signaling processes.

Document Type: Article
Additional Information: Times Cited: 1
Research affiliation: Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > The Future Ocean - Cluster of Excellence > FO-R04
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1128/aem.03290-14
ISSN: 0099-2240
Projects: Future Ocean
Date Deposited: 18 Oct 2016 03:46
Last Modified: 23 Sep 2019 19:44
URI: http://oceanrep.geomar.de/id/eprint/32748

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