,Histological features POF I,"The POF has a clear convoluted shape and a rather big lumen. It consists of two cell layers, theca and granulosa with clearly visible nuclei and relatively thin width with similar morphology to the follicle wall in advanced vitellogenic oocytes. The cross sectional area is larger compared to that of other identified POF stages. This type of POF occurred exclusively in ovaries where vitellogenesis was the most advance stage of oocyte development (Fig. 3a)." POF II,"The POF has still a distinct convoluted shape but the lumen has become smaller. The two cell layers are still distinguishable but first signs of degeneration are visible: Vacuoles become visible and nuclear pycnosis occurs. The thickness of the two cell layers has clearly increased and granulosa cells are more rectangular. Again, this type of POF occurred exclusively in ovaries where oocytes have not developed further than vitellogenic stage (Fig. 3b)." POF III,"The convoluted shape begins to disappear, as the follicle is being resorbed. The POF lumen shrinks and is no longer clearly distinct. It is not possible to distinguish between the two cell layers. The cross sectional area has clearly decreased compared to the two first POF stages. The thickness of the two cell layer has decreased again due to degeneration. This POF type occurred in ovaries with early and advanced germinal vesicle migration stage or in ovaries with early hydrated oocytes (Fig. 3c)." POF IV,"The convoluted shape and lumen are not visible anymore. The follicle has shrunk considerably, becoming more compact and the cross sectional area is very small compared to the other stages. This POF type occurred in ovaries with advanced germinal vesicle migration and hydrated oocytes (Fig. 3d). Remains of this stage are also detectable in vitellogenic ovaries with very recent POFs." Haul,Date,Time,n,Sex ratio,Fraction of: Vitellogenesis,GVM,Hydrated,POF,POF,POF,POF,No POF I,II,III,IV 1,16 April 2007,06:18,21,0.41,0.19,0.57,0.24,0.00,0.10,0.38,0.43,0.10 2,16 April 2007,09:00,23,0.48,0.30,0.57,0.13,0.09,0.17,0.35,0.30,0.09 3,16 April 2007,12:00,23,0.22,0.86,0.14,0.00,0.65,0.22,0.09,0.00,0.04 4,16 April 2007,15:00,20,0.22,0.50,0.10,0.40,0.25,0.25,0.05,0.40,0.05 5,16 April 2007,18:00,20,0.31,0.35,0.15,0.50,0.20,0.10,0.15,0.55,0.00 6,16 April 2007,21:00,20,0.58,0.50,0.25,0.25,0.10,0.25,0.35,0.20,0.10 7,17 April 2007,00:00,21,0.54,0.38,0.38,0.24,0.14,0.19,0.38,0.24,0.05 8,17 April 2007,03:00,21,0.30,0.38,0.48,0.14,0.29,0.10,0.38,0.19,0.05 9,17 April 2007,06:00,21,na,0.24,0.57,0.19,0.10,0.10,0.52,0.29,0.00