Production of scopularide A in submerged culture with Scopulariopsis brevicaulis.

Tamminen, Anu, Kramer, Annemarie, Labes, Antje and Wiebe, Marilyn G. (2014) Production of scopularide A in submerged culture with Scopulariopsis brevicaulis. Open Access Microbial Cell Factories, 13 (1). p. 89. DOI 10.1186/1475-2859-13-89.

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Background: Marine organisms produce many novel compounds with useful biological activity, but are currently underexploited. Considerable research has been invested in the study of compounds from marine bacteria, and several groups have now recognised that marine fungi also produce an interesting range of compounds. During product discovery, these compounds are often produced only in non-agitated culture conditions, which are unfortunately not well suited for scaling up. A marine isolate of Scopulariopsis brevicaulis, strain LF580, produces the cyclodepsipeptide scopularide A, which has previously only been produced in non-agitated cultivation.

Results: Scopulariopsis brevicaulis LF580 produced scopularide A when grown in batch and fed-batch submerged cultures. Scopularide A was extracted primarily from the biomass, with approximately 7% being extractable from the culture supernatant. By increasing the biomass density of the cultivations, we were able to increase the volumetric production of the cultures, but it was important to avoid nitrogen limitation. Specific production also increased with increasing biomass density, leading to improvements in volumetric production up to 29-fold, compared with previous, non-agitated cultivations. Cell densities up to 36 g L-1 were achieved in 1 to 10 L bioreactors. Production of scopularide A was optimised in complex medium, but was also possible in a completely defined medium.

Conclusions: Scopularide A production has been transferred from a non-agitated to a stirred tank bioreactor environment with an approximately 6-fold increase in specific and 29-fold increase in volumetric production. Production of scopularide A in stirred tank bioreactors demonstrates that marine fungal compounds can be suitable for scalable production, even with the native production organism.

Document Type: Article
Funder compliance: info:eu-repo/grantAgreement/EC/FP7/265926
Additional Information: WOS:000338263700001
Keywords: Marine fungi; Scopulariopsis brevicaulis; Scopularide A; Stirred tank bioreactor
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry
OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > GEOMAR > Applied R&D > Centre for Marine Substances (KiWiZ)
Refereed: Yes
Open Access Journal?: Yes
Publisher: BioMed Central
Projects: Marine Fungi, KIWIZ, Future Ocean
Date Deposited: 23 Jun 2014 12:24
Last Modified: 23 Sep 2019 20:29

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