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Chemical analysis of the Alphaproteobacterium strain MOLA1416 associated with the marine lichen Lichina pygmaea.
Parrot, Delphine, Intertaglia, Laurent, Jehan, Philippe, Grube, Martin, Suzuki, Marcelino T. and Tomasi, Sophie (2018) Chemical analysis of the Alphaproteobacterium strain MOLA1416 associated with the marine lichen Lichina pygmaea. Phytochemistry, 145 . pp. 57-67. DOI 10.1016/j.phytochem.2017.10.005.
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Abstract
Alphaproteobacterium strain MOLA1416, related to Mycoplana ramosa DSM 7292 and Chelativorans intermedius CC-MHSW-5 (93.6% 16S rRNA sequence identity) was isolated from the marine lichen, Lichina pygmaea and its chemical composition was characterized by a metabolomic network analysis using LC-MS/MS data. Twenty-five putative different compounds were revealed using a dereplication workflow based on MS/MS signatures available through GNPS (https://gnps.ucsd.edu/). In total, ten chemical families were highlighted including isocoumarins, macrolactones, erythrinan alkaloids, prodiginines, isoflavones, cyclohexane-diones, sterols, diketopiperazines, amino-acids and most likely glucocorticoids. Among those compounds, two known metabolites (13 and 26) were isolated and structurally identified and metabolite 26 showed a high cytotoxic activity against B16 melanoma cell lines with an IC50 0.6 ± 0.07 μg/mL.
Document Type: | Article |
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Keywords: | Lichen-associated bacteria; Lichina pygmaea; Alphaproteobacteria; LC/MSn; Dereplication workflow; Molecular networking |
Research affiliation: | OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry |
Refereed: | Yes |
Open Access Journal?: | No |
Publisher: | Elsevier |
Date Deposited: | 22 Nov 2017 10:36 |
Last Modified: | 08 Feb 2021 07:31 |
URI: | https://oceanrep.geomar.de/id/eprint/40207 |
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