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Boron isotope variability related to boron speciation (change during uptake and transport) in bell pepper plants and SI traceable n ( 11 B)/ n ( 10 B) ratios for plant reference materials.
Geilert, Sonja , Vogl, Jochen, Rosner, Martin and Eichert, Thomas (2019) Boron isotope variability related to boron speciation (change during uptake and transport) in bell pepper plants and SI traceable n ( 11 B)/ n ( 10 B) ratios for plant reference materials. Rapid Communications in Mass Spectrometry, 33 (13). pp. 1137-1147. DOI 10.1002/rcm.8455.
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Abstract
Rationale
Boron (B) is an essential micronutrient in plants and its isotope variations are used to gain insights into plant metabolism, which is important for crop plant cultivation. B isotope variations were used to trace intraplant fractionation mechanisms in response to the B concentration in the irrigation water spanning the range from B depletion to toxic levels.
Methods
A fully validated analytical procedure based on MC‐ICP‐MS, sample decomposition and B matrix separation was applied to study B isotope fractionation. The validation was accomplished by establishing a complete uncertainty budget and by applying reference materials, yielding expanded measurement uncertainties of 0.8 ‰ for pure boric acid solutions and ≤ 1.5 ‰ for processed samples. With this validated procedure SI traceable B isotope amount ratios were determined in plant reference materials for the first time.
Results
The B isotope compositions of irrigation water and bell pepper samples suggest passive diffusion of the heavy 11B isotope into the roots during low to high B concentrations while uptake of the light 10B isotope was promoted during B depletion, probably by active processes. A systematic enrichment of the heavy 11B isotope in higher located plant parts was observed (average Δ11Bleaf‐roots = 20.3 ± 2.8 ‰ (1 SD)), possibly by a facilitated transport of the heavy 11B to growing meristems by B transporters.
Conclusions
B isotopes can be used to identify plant metabolism in response to the B concentration in the irrigation water and during intraplant B transfer. The large B isotope fractionation within the plants demonstrates the importance of biological B cycling for the global B cycle.
Document Type: | Article |
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Keywords: | CHANNEL-MEDIATED TRANSPORT; BORIC-ACID; RHAMNOGALACTURONAN-II; ARABIDOPSIS-THALIANA; STABLE-ISOTOPES; STRUCTURAL ROLE; FRACTIONATION; SQUASH; TRANSLOCATION; MECHANISMS |
Research affiliation: | OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-MG Marine Geosystems |
Refereed: | Yes |
Open Access Journal?: | No |
Publisher: | Wiley |
Date Deposited: | 24 Apr 2019 12:40 |
Last Modified: | 31 Jan 2022 09:23 |
URI: | https://oceanrep.geomar.de/id/eprint/46422 |
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