Enzymatische in situ Messungen - neue Meßmethoden für Meerwasser und Sediment.

Gradl, Toni (1974) Enzymatische in situ Messungen - neue Meßmethoden für Meerwasser und Sediment. Open Access Kieler Meeresforschungen, 30 (1). pp. 1-11.

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Abstract

1.1. A percolator was constructed to measure continously metabolic processes in sediments. A continous flow of water penetrates the sediment. Oxygen can be excluded. A continous polarographic control is possible or a control with enzyme electrodes. Gases evolved by metabolism can be trapped. It was attempted to discriminate bacterial and purely enzymatic processes (free enzymes) by addition of chloroform or toluene, both of which will kill bacteria. The attempt was unsuccessful.
1.2. Enzyme electrodes were constructed as a probe for sediments, for the percolator, and for the free water. The enzyme electrodes permit the determination of the ratios of inorganic phosphorus/organic phosphorus, urea/ammonia, glycosidically bound glucose/free glucose. The enzyme is coupled to a diazotized anilin resin which is fixed on a metal electrode as a thin layer. The potentials (measured against Ag-AgCl) correlate with the ratio of the concentrations of substrate and product. It is shown, how absolute measurements can be performed. The electrodes are calibrated in Tris-HCl-buffer solutions pH 8.
1.3. To study the fine structure of sediments matrix bound colour reactions are used. The matrices are fixed to glass slides.Glucose is bound glycosidically to an epoxy resin. After exposition the remaining glucose is measured by a colour reaction. Dehydrogenases are detected by 2, 3, 5 triphenyltetrazoliumchlorid which is fixed to a matrix by spraying with a teflon aerosol. The yellow formazan colour is measured. Hydrogen sulfide is detected by lead acetate which is enclosed in the cells of a dried emulsion. The walls are permeable for gas (H2S), but not for the insoluble reaction product. All reactions are calibrated in Tris-HCl-buffer solutions pH 8.

Document Type: Article
Additional Information: Printausgabe in Bibliothek vorhanden - print edition available
Refereed: No
Open Access Journal?: Yes
Publisher: Institut für Meereskunde
Date Deposited: 17 May 2022 09:22
Last Modified: 17 May 2022 09:22
URI: https://oceanrep.geomar.de/id/eprint/56013

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