Andersen Elvehøy, I. L. and Thingstad, T. (1991) Detection of limiting factors for bacterial activity using protease activity. Kieler Meeresforschungen - Sonderheft, 8 . pp. 392-398.

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Abstract

Responses in protease activity, caused by adding nutrients to batch cultures of a Vibrio sp., grown to equilibria in a chemostat system at 5 °C with C, N or P-limited media, was measured after O, 6 - 8 and 24 C±) hrs. Addition of the limiting nutrient gave the largest response in activity, due to an increase in bacterial numbers. Reduction in activity per cell was, however, observed in some cases. In field samples from the Skagerak, clear responses within 24 hrs were either absent or found when both C, N and P-sources were added. In the field samples, less changes were found in activity per cell. At three coastal stations, detectable responses were found to additions of P04 3- alone, or in some combinations. When measured, leucine incorporation gave a response pattern to P04 3- additions similar to that of protease activity.

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