Cisternas-Nuevo, C., Lee, C. and Engel, Anja (2011) Measurements of proteinaceous and polysaccharide-rich particles produced by bubbling phytoplankton exduates. [Poster] In: ASLO Aquatic Sciences Meeting. , 13.-18.02.2011, San Juan, Puerto Rico, USA .

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Abstract

Transparent stainable particles (TSP) were produced in the laboratory by bubbling dissolved exudates released by the diatom Thalassiosira pseudonana. We developed a method to quantify proteinaceous TSP, identified by staining with Coomassie Brilliant Blue and called Coomassie stainable particles (CSP). This method is similar to that used to quantify polysaccharide-rich TSP, identified by staining with Alcian Blue and known as transparent exopolymeric particles (TEP). Measurement of CSP is colorimetric and is quantified relative to a particulate bovine serum albumin standard (in a manner similar to how TEP is quantified relative to xanthan gum). Although this research demonstrates that coagulation of dissolved phytoplankton exudate produces TSP that contain both polysaccharides and proteins, but leaves open the question of whether protein is absorbed onto TEP (as suggested previously) or forms discrete particles. Understanding the chemical composition of TSP would aid in determining sources of these particles in the ocean and their role in biogeochemical process such as aggregation. A colorimetric method to quantify CSP allows faster and more quantitative measurements, providing an efficient tool to investigate their role in seawater.

Document Type:	Conference or Workshop Item (Poster)
Keywords:	Marine Biology; phytoplankton
Research affiliation:	OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Date Deposited:	11 Oct 2011 10:58
Last Modified:	15 Mar 2012 12:20
URI:	https://oceanrep.geomar.de/id/eprint/12322

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