Broenneke, Simone, Brueckner, Bodo, Peters, Nils, Bosch, Thomas, Staeb, Franz, Wenck, Horst, Hagemann, Sabine and Winnefeld, Marc (2012) DNA methylation regulates lineage-specifying genes in primary lymphatic and blood endothelial cells. Angiogenesis, 15 (2). pp. 317-329. DOI 10.1007/s10456-012-9264-2.

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Abstract

During embryonic development, the lymphatic system emerges by transdifferentiation from the cardinal vein. Although lymphatic and blood vasculature share a close molecular and developmental relationship, they display distinct features and functions. However, even after terminal differentiation, transitions between blood endothelial cells (BEC) and lymphatic endothelial cells (LEC) have been reported. Since phenotypic plasticity and cellular differentiation processes frequently involve epigenetic mechanisms, we hypothesized that DNA methylation might play a role in regulating cell type-specific expression in endothelial cells. By analyzing global gene expression and methylation patterns of primary human dermal LEC and BEC, we identified a highly significant set of genes, which were differentially methylated and expressed. Pathway analyses of the differentially methylated and upregulated genes in LEC revealed involvement in developmental and transdifferentiation processes. We further identified a set of novel genes, which might be implicated in regulating BEC-LEC plasticity and could serve as therapeutic targets and/or biomarkers in vascular diseases associated with alterations in the endothelial phenotype.

Document Type:	Article
Keywords:	Primary human endothelial cells Gene expression DNA methylation Transdifferentiation Endothelial plasticity Biomarker
Research affiliation:	Kiel University OceanRep > The Future Ocean - Cluster of Excellence
Refereed:	Yes
Open Access Journal?:	No
Publisher:	American Medical Associa
Projects:	Future Ocean
Date Deposited:	14 May 2014 10:17
Last Modified:	23 Sep 2019 23:49
URI:	https://oceanrep.geomar.de/id/eprint/23874

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