Ilmberger, N., Meske, D., Juergensen, J., Schulte, M., Barthen, P., Rabausch, U., Angelov, A., Mientus, M., Liebl, W., Schmitz, Ruth A. and Streit, W. R. (2012) Metagenomic cellulases highly tolerant towards the presence of ionic liquids-linking thermostability and halotolerance. Applied Microbiology and Biotechnology, 95 (1). pp. 135-146. DOI 10.1007/s00253-011-3732-2.

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Abstract

Cellulose is an important renewable resource for the production of bioethanol and other valuable compounds. Several ionic liquids (ILs) have been described to dissolve water-insoluble cellulose and/or wood. Therefore, ILs would provide a suitable reaction medium for the enzymatic hydrolysis of cellulose if cellulases were active and stable in the presence of high IL concentrations. For the discovery of novel bacterial enzymes with elevated stability in ILs, metagenomic libraries from three different hydrolytic communities (i.e. an enrichment culture inoculated with an extract of the shipworm Teredo navalis, a biogas plant sample and elephant faeces) were constructed and screened. Altogether, 14 cellulolytic clones were identified and subsequently assayed in the presence of six different ILs. The most promising enzymes, CelA2, CelA3 (both derived from the biogas plant) and CelA84 (derived from elephant faeces), showed high activities (up to 6.4 U/mg) in the presence of 30% (v/v) ILs. As these enzymes were moderately thermophilic and halotolerant, they retained 40% to 80% relative activity after 34 days in 4 M NaCl, and they were benchmarked with two thermostable enzymes, CelA from Thermotoga maritima and Cel5K from a metagenome library derived from Avachinsky crater in Kamchatka. These enzymes also exhibited high activity (up to 11.1 U/mg) in aqueous IL solutions (30% (v/v)). Some of the enzymes furthermore exhibited remarkable stability in 60% (v/v) IL. After 4 days, CelA3 and Cel5K retained up to 79% and 100% of their activity, respectively. Altogether, the obtained data suggest that IL tolerance appears to correlate with thermophilicity and halotolerance.

Document Type:	Article
Additional Information:	Univ Hamburg, Biozentrum Klein Flottbek, Abt Mikrobiol & Biotechnol, D-22609 Hamburg, Germany. Univ Kiel, Inst Allgemeine Mikrobiol, D-24118 Kiel, Germany. Merck KGaA, PM ABE R&D S&TS, Ion Liquids Res Lab, D-64293 Darmstadt, Germany. Tech Univ Munich, Lehrstuhl Mikrobiol, D-85354 Freising Weihenstephan, Germany. Streit, WR (reprint author), Univ Hamburg, Biozentrum Klein Flottbek, Abt Mikrobiol & Biotechnol, Ohnhorststr 18, D-22609 Hamburg, Germany. Wolfgang.Streit@uni-hamburg.de
Keywords:	Cellulase Ionic liquid Metagenomics Halotolerance Thermostability pyrrolidone carboxyl peptidase clostridium-thermocellum cellobiohydrolase cbha pyrococcus-furiosus stability libraries cellulose hyperthermophile construction dissolution
Research affiliation:	Kiel University Kiel University > Kiel Marine Science OceanRep > The Future Ocean - Cluster of Excellence OceanRep > The Future Ocean - Cluster of Excellence > FO-R04
Refereed:	Yes
Open Access Journal?:	No
Publisher:	American Medical Association
Projects:	Future Ocean
Date Deposited:	14 May 2014 10:00
Last Modified:	23 Sep 2019 22:26
URI:	https://oceanrep.geomar.de/id/eprint/24047

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