Sun, W., Shi, Y., Seekamp, A., Gorb, Stanislav, Motta, A., Migliaresi, C. and Fuchs, S. (2014) Co-culture system of outgrowth endothelial cells with mesenchymal stem cells in silk fibroin hydrogel enhances angiogenesis independent from the conjugation of IKVAV peptide. Journal of Tissue Engineering and Regenerative Medicine, 8 . pp. 193-194. DOI 10.1088/1748-6041/11/3/035009.

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Abstract

Sufficient vascularization of the implant construct is required for tissue regeneration to ensure the supply of oxygen and nutrients. In our previous work, we established sonication-induced silk fibroin hydrogel to load neural stem cells for brain tissue engineering applications. In this study, we explored the application of silk fibroin as an injectable hydrogel for vascularization of soft tissues. We investigated the ability of outgrowth endothelial cells (OECs) in mono-culture or in co-culture with human bone marrow-derived mesenchymal stem cells (BM-MSCs) to form capillary networks in silk fibroin hydrogels. Furthermore, the silk fibroin hydrogel was modified with IKVAV peptide revealing a sequence derived from the extracellular matrix component laminin-1 to test its effects on angiogenesis, using unmodified and VVIAK modified silk fibroin hydrogel as controls. In monocultures of OECs, no angiogenic structures were observed in silk fibroin hydrogels. In contrast, vascular structures were abundant and increased in co-culture, as confirmed by immunocytochemistry and scanning electron microscopy (SEM) over 10 d of culture in silk fibroin-based hydrogels. Although no significant differences in angiogenic activity seem to be caused by the IKVAV peptide in our experimental settings, these results indicate that sonication-induced silk fibroin-based hydrogels support the formation of functional endothelial tubes and vascularization networks in the presence of mesenchymal cells supporting the vascular sprouting of endothelial cells.

Document Type:	Article
Additional Information:	Times Cited: 0 SI 1 0
Research affiliation:	Kiel University > Kiel Marine Science OceanRep > The Future Ocean - Cluster of Excellence Kiel University
Refereed:	Yes
Open Access Journal?:	No
Publisher:	IOP Science
Projects:	Future Ocean
Date Deposited:	30 Mar 2015 12:47
Last Modified:	23 Aug 2019 09:15
URI:	https://oceanrep.geomar.de/id/eprint/28247

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