Kumar, Abhishek, Henrissat, Bernard, Arvas, Mikko, Syed, Muhammad Fahad, Thieme, Nils, Benz, J. Philipp, Sorensen, Jens Laurids, Record, Eric, Poeggeler, Stefanie and Kempken, Frank (2015) De Novo Assembly and Genome Analyses of the Marine-Derived Scopulariopsis brevicaulis Strain LF580 Unravels Life-Style Traits and Anticancerous Scopularide Biosynthetic Gene Cluster. PLoS ONE, 10 (10). DOI 10.1371/journal.pone.0140398.

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Abstract

The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA sequencing methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb and 16298 putative gene models. We identified a large non-ribosomal peptide synthetase gene (nrps1) and supporting pks2 gene in the same biosynthetic gene cluster. This cluster and the genes within the cluster are functionally active as confirmed by RNA-Seq. Characterization of carbohydrate-active enzymes and major facilitator superfamily (MFS)-type transporters lead to postulate S. brevicaulis originated from a soil fungus, which came into contact with the marine sponge Tethya aurantium. This marine sponge seems to provide shelter to this fungus and micro-environment suitable for its survival in the ocean. This study also builds the platform for further investigations of the role of life-style and secondary metabolites from S. brevicaulis.

Document Type:	Article
Additional Information:	Times Cited: 1
Research affiliation:	OceanRep > The Future Ocean - Cluster of Excellence OceanRep > The Future Ocean - Cluster of Excellence > FO-R03 Kiel University
Refereed:	Yes
Open Access Journal?:	No
Publisher:	Public Library of Science
Projects:	Future Ocean
Date Deposited:	18 Oct 2016 03:45
Last Modified:	23 Sep 2019 16:54
URI:	https://oceanrep.geomar.de/id/eprint/32562

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