Andrews, Kimberly R., Good, Jeffrey M., Miller, Michael R., Luikart, Gordon and Hohenlohe, Paul A. (2016) Harnessing the power of RADseq for ecological and evolutionary genomics. Nature Reviews Genetics, 17 (2). pp. 81-92. DOI 10.1038/nrg.2015.28.

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Abstract

High-throughput techniques based on restriction site-associated DNA sequencing
(RADseq) are enabling the low-cost discovery and genotyping of thousands of genetic markers
for any species, including non-model organisms, which is revolutionizing ecological, evolutionary
and conservation genetics. Technical differences among these methods lead to important
considerations for all steps of genomics studies, from the specific scientific questions that can be
addressed, and the costs of library preparation and sequencing, to the types of bias and error
inherent in the resulting data. In this Review, we provide a comprehensive discussion of RADseq
methods to aid researchers in choosing among the many different approaches and avoiding
erroneous scientific conclusions from RADseq data, a problem that has plagued other genetic
marker types in the past.

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