Yang, W. T., Rosenstiel, Philip and Schulenburg, Hinrich (2016) ABSSeq: a new RNA-Seq analysis method based on modelling absolute expression differences. BMC Genomics, 17 . DOI 10.1186/s12864-016-2848-2.

Full text not available from this repository.

Abstract

Background: The recent advances in next generation sequencing technology have made the sequencing of RNA (i.e., RNA-Seq) an extemely popular approach for gene expression analysis. Identification of significant differential expression represents a crucial initial step in these analyses, on which most subsequent inferences of biological functions are built. Yet, for identification of these subsequently analysed genes, most studies use an additional minimal threshold of differential expression that is not captured by the applied statistical procedures. Results: Here we introduce a new analysis approach, ABSSeq, which uses a negative binomal distribution to model absolute expression differences between conditions, taking into account variations across genes and samples as well as magnitude of differences. In comparison to alternative methods, ABSSeq shows higher performance on controling type I error rate and at least a similar ability to correctly identify differentially expressed genes. Conclusions: ABSSeq specifically considers the overall magnitude of expression differences, which enhances the power in detecting truly differentially expressed genes by reducing false positives at both very low and high expression level. In addition, ABSSeq offers to calculate shrinkage of fold change to facilitate gene ranking and effective outlier detection.

Document Type:	Article
Additional Information:	Times Cited: 0 Yang, Wentao Rosenstiel, Philip C. Schulenburg, Hinrich
Keywords:	RNA-Seq, Transcriptome Analysis, Differential gene Expression, ABSSeq, Negative binomial Distribution
Research affiliation:	Kiel University > Kiel Marine Science OceanRep > The Future Ocean - Cluster of Excellence Kiel University
Refereed:	Yes
Open Access Journal?:	No
Publisher:	BioMed Central
Projects:	Future Ocean
Date Deposited:	20 Feb 2017 11:49
Last Modified:	17 May 2019 11:59
URI:	https://oceanrep.geomar.de/id/eprint/36382

Actions (login required)

View Item