Tholey, Andreas and Becker, Alexander (2017) Top-down proteomics for the analysis of proteolytic events - Methods, applications and perspectives. Biochimica et Biophysica Acta - Molecular Cell Research, 1864 (11). pp. 2191-2199. DOI 10.1016/j.bbamcr.2017.07.002.

Full text not available from this repository.

Abstract

Mass spectrometry based proteomics is an indispensable tool for almost all research areas relevant for the understanding of proteolytic processing, ranging from the identification of substrates, products and cleavage sites up to the analysis of structural features influencing protease activity. The majority of methods for these studies are based on bottom-up proteomics performing analysis at peptide level. As this approach is characterized by a number of pitfalls, e.g. loss of molecular information, there is an ongoing effort to establish top-down proteomics, performing separation and MS analysis both at intact protein level. We briefly introduce major approaches of bottom-up proteomics used in the field of protease research and highlight the shortcomings of these methods. We then discuss the present state-of-the-art of top-down proteomics. Together with the discussion of known challenges we show the potential of this approach and present a number of successful applications of top-down proteomics in protease research. This article is part of a Special Issue entitled: Proteolysis as a Regulatory Event in Pathophysiology edited by Stefan Rose-John.

Document Type:	Article
Keywords:	C-terminomics; Degradomics; Mass spectrometry; N-terminomics; Posttranslational modification
Research affiliation:	Kiel University > Kiel Marine Science OceanRep > The Future Ocean - Cluster of Excellence
Refereed:	Yes
Open Access Journal?:	No
Publisher:	Elsevier
Date Deposited:	08 Jan 2018 10:40
Last Modified:	13 May 2019 11:11
URI:	https://oceanrep.geomar.de/id/eprint/41244

Actions (login required)

View Item