Lei, Benfang, Brinkmann, Julia, Koudelka, Tomas, Keppler, Julia K., Tholey, Andreas, Schwarz, Karin, Thaller, Georg and Tetens, Jens (2015) Characterization of an Equine α-S2-Casein Variant Due to a 1.3 kb Deletion Spanning Two Coding Exons. PLoS ONE, 10 (10). e0139700. DOI 10.1371/journal.pone.0139700.

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Abstract

The production and consumption of mare’s milk in Europe has gained importance, mainly based on positive health effects and a lower allergenic potential as compared to cows’ milk. The allergenicity of milk is to a certain extent affected by different genetic variants. In classical dairy species, much research has been conducted into the genetic variability of milk proteins, but the knowledge in horses is scarce. Here, we characterize two major forms of equine αS2-casein arising from genomic 1.3 kb in-frame deletion involving two coding exons, one of which represents an equid specific duplication. Findings at the DNA-level have been verified by cDNA sequencing from horse milk of mares with different genotypes. At the protein-level, we were able to show by SDS-page and in-gel digestion with subsequent LC-MS analysis that both proteins are actually expressed. The comparison with published sequences of other equids revealed that the deletion has probably occurred before the ancestor of present-day asses and zebras diverged from the horse lineage.

Document Type:	Article
Research affiliation:	Kiel University > Kiel Marine Science OceanRep > The Future Ocean - Cluster of Excellence
Refereed:	Yes
Open Access Journal?:	Yes
Publisher:	Public Library of Science
Date Deposited:	01 Feb 2018 12:37
Last Modified:	26 Apr 2019 11:09
URI:	https://oceanrep.geomar.de/id/eprint/41789

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