Klimovich, Alexander, Wittlieb, Jörg and Bosch, T. C. G. (2019) Transgenesis in Hydra to characterize gene function and visualize cell behavior. Nature Protocols, 14 (7). pp. 2069-2090. DOI 10.1038/s41596-019-0173-3.

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Abstract

The freshwater polyp Hydra is a cnidarian used as a model organism in a number of fields, including the study of the origin and evolution of developmental mechanisms, aging, symbiosis and host–microbe interactions. Here, we describe a procedure for the establishment of stable transgenic Hydra lines by embryo microinjection. The three-stage protocol comprises (i) the design and preparation of a transgenic construct, (ii) the microinjection of the vector into early embryos of Hydra vulgaris, and (iii) the selection and enrichment of mosaic animals in order to develop uniformly transgenic clonal lines. The preparation of a transgenic construct requires ~2 weeks, and transgenic lines can be obtained within 3 months. The method allows constitutive or inducible gain- and loss-of-function approaches, as well as in vivo tracing of individual cells. Hydra polyps carrying transgenic cells reveal functional properties of the ancestral circuitry controlling animal development.

Document Type:	Article
Research affiliation:	Kiel University > Kiel Marine Science OceanRep > The Future Ocean - Cluster of Excellence Kiel University
Refereed:	Yes
Open Access Journal?:	No
Publisher:	Nature Research
Projects:	Future Ocean
Date Deposited:	01 Aug 2019 12:26
Last Modified:	02 Jan 2020 12:12
URI:	https://oceanrep.geomar.de/id/eprint/47319

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