Meyer-Reil, Lutz-Arend (1975) An improved method for the semicontinuous culture of bacterial populations of Nuclepore membrane filters. Kieler Meeresforschungen, 31 (1). pp. 1-6.

Preview

Text Meyer-Reil_L_1975.pdf - Reprinted Version Available under License Creative Commons: Attribution 3.0. Download (786kB) | Preview

Abstract

The present paper describes an improved method for the semicontinuous culture of bacterial populations on Nuclepore membrane filters. The filters, placed on swimming rings, are incubated in direct contact with the water surface of the flow system, and examined by fluorescence microscopy. The growth activity of both, pure cultures and natural bacterial populations, measured in the flow system is compared to the activity measured in a conventional culture system (incubation of membrane filters on the surface of paper disc saturated with incubation medium). The flow system increases the number and the spectrum of colony forming cells. The reasons for the superiority of the flow system are discussed.

Actions (login required)

View Item