Schulz, D., Passeri, A., Schmidt, M., Lang, S., Wagner, F., Wray, V., Poremba, K. and Gunkel, W. (1991) Screening for biosurfactants among crude oil degrading marine microorganisms. Kieler Meeresforschungen - Sonderheft, 8 . pp. 322-326.

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Abstract

Cellbound and extracellular biosurfactants were detected by thin-layer chromatography, tensiometric measurements, blood agar lysis and their capability of stabilizing oil in water emulsions. Known trehalose lipids (RAPP et al. 1979, RISTAU et al. 1983) and one glucose lipid, formerly unknown, with excellent properties were isolated. Also emulsifying agents were found. They seemed to be high molecular weight substances. Mixtures of hydrocarbons containing aliphatics and aromatics were efficiently emulsified. Reduced activity after digestion by several proteases gives a first indication of the nature of the emulsifiers. Preliminary results with several marine microorganisms showed that most biogenic surfactants possess a good environmental compatibility (v. BERNEM 1984, LANG et al. 1986).

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