Nagel, Kerstin (2013) Naturstoffe algenassoziierter Bakterien & Naturstoffe der Gattung Pseudomonas und deren Bedeutung für die Braunalge Saccharina latissima. (PhD/ Doctoral thesis), Christian-Albrechts-Universität Kiel, Kiel, Germany, 177 pp.

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Abstract

An association between strains of Bacillus, Pseudomonas and Streptomyces with the brown alga Saccharina latissima from the Baltic Sea was shown by the results of this thesis. In particular, a mutualistic symbiosis is assumed for Pseudomonas strain LD120 and S. latissima, due to metabolic characteristics and the secondary metabolite profile. Members of the genera Bacillus, Kiloniella, Pseudomonas, Pseudoalter-omonas and Streptomyces, all isolated from S. latissima, produced bioactive compounds. Variations of cultivation conditions lead to different sec-ondary metabolite profiles of these bacteria. Particularly noteworthy is the production of six new compounds by strain LD81 after addition of the biogenic amine putrescine to the culture medium. Bioactivity of the isolated bacterial natural products comprises antibacterial and antifugal activity, as well as inhibition of human cancer cell lines and acetylcholinesterase. Approximately half of the 134 identified compounds was dereplicated and assigned to compounds already known from literature. However, two compounds (holomycin and N-propionylholothin) were isolated for the first time from the genus Pseudomonas. Potentially novel bioactive compounds were identified from members of the genera Bacillus (LB084, m/z 572), Pseudomonas (LD120, m/z 347) and Streptomyces (LB058, m/z 544). In addition, derivatives of known compounds were identified in at least two strains.

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