Iftime, Dumitrita, Jasyk, Martin, Kulik, Andreas, Imhoff, Johannes F. , Stegmann, Evi, Wohlleben, Wolfgang, Süssmuth, Roderich D. and Weber, Tilmann (2015) Streptocollin, a Type IV Lanthipeptide Produced by Streptomyces collinus Tü 365. ChemBioChem, 16 (18). pp. 2615-2623. DOI 10.1002/cbic.201500377.

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Abstract

Lanthipeptides are ribosomally synthesized and post-translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5- and 5.5-fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster.

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